| 當期課號 |
7231 |
Course Number |
7231 |
| 授課教師 |
李孟真 |
Instructor |
LEE,MENG JEN |
| 中文課名 |
進階細胞學技術 |
Course Name |
Cell Biology Technologies for Advanced Study |
| 開課單位 |
生化科技研究所博士班二A |
Department |
|
| 修習別 |
選修 |
Required/Elective |
Elective |
| 學分數 |
3 |
Credits |
3 |
| 課程目標 |
生物醫學之研究常使用活體外細胞培養之技術 ，期望可了解分子層級的機制。 研究生執行論文實驗計畫前應該對相關種種技術之理論及實際操作面多加了解。本課程希望提供即將從事進階生物醫學相關計畫之不同學習背景的博士班學生的訓練。 |
Objectives |
In vitro cultures of the cells were frequently used in biomedical research for the study of mechanism at the molecular level. Graduate students should be equipped with knowledge of this technology before carrying out their research project. This course is designed for training PhD students of different background that would take on advanced study in the field of biomedical research. |
| 教材 |
Culture of animal cells: a manual of basic technique and specialized application. by R Freshney. published by Wiley inc. |
Teaching Materials |
Culture of animal cells: a manual of basic technique and specialized application. by R Freshney. published by Wiley inc. |
| 成績評量方式 |
考試及平常成績 |
Grading |
test and presence |
| 教師網頁 |
|
| 教學內容 |
相關理論 實驗室 無菌操作 氣體 培養基
細胞株一般培養
分離細胞株
不同 分子與胞器的分離
細胞生長與轉化
汙染源
定量及試驗
初代細胞培養 (一)
初代細胞培養 (二)
組織切片染色技術
免疫螢光染色技術
血球細胞的分離
流式細胞儀分析 |
Syllabus |
the theoretical aspects, the lab layout, aseptic techniques, the gas, the media
maintenance of the cell lines
cloning and selection of cells
physical methods of cell separation
cell growth and transformation
contmination
quantification and experimental design
culture of primary cells (1)
culture of primary cells (2)
histology
immunostaining
separation of blood cells
flow cytometry |