當期課號 |
7628 |
Course Number |
7628 |
授課教師 |
羅致逑 |
Instructor |
LO,,CHI CHU |
中文課名 |
分離技術特論 |
Course Name |
Advance Techniques for Separation |
開課單位 |
應用化學系碩士在職專班一A |
Department |
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修習別 |
選修 |
Required/Elective |
Elective |
學分數 |
3 |
Credits |
3 |
課程目標 |
使同學了解化學分析時,有那些分離技術可應用,以達到化學物質與生技產品的分析,鑑定。 |
Objectives |
To introduce the concepts and the techniqes used in separation for the analyses and identifications of chemical compounds and biotech products . |
教材 |
儀器分析(Principles of Instrumental Analysis) |
Teaching Materials |
|
成績評量方式 |
上課表現(30%),考試成績(70%) |
Grading |
Class performance(30%),test(70%) |
教師網頁 |
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教學內容 |
使同學了解化學分析時,有那些分離技術可應用,以達到化學物質與生技產品的分析,鑑定。 全課程分五章,分別為第一章:層析分離緒論;第二章:氣相層析法;第三章:高效能液相層析法;第四章:毛細管電泳法和毛細管電層析法;第五章:聚合酶連鎖反應的理論與應用。 第一章:層析分離緒論 1.層析法概述;2.溶質的移動速率;3.區帶變寬與管柱效率;4.管柱效能的最適化;5.層析法重要關係式的摘要;6.層析法的應用。 第二章:氣相層析法 1.氣相-液相層析法的原理;2.氣相層析法管柱與靜相;3.氣相-液相層析的應用。 期中考 第三章:高效能液相層析法 1.HPLC的原理;2.液相層析法的管柱效率;3.分配層析法 第四章:1.毛細管電泳法和毛細管電層析法;2.毛細管電泳法;3.毛細管電泳的應用 第五章:PCR 1.聚合酶連鎖反應的理論;2.應用 期未考 |
Syllabus |
To introduce the concepts and the techniqes used in separation for the analyses and identifications of chemical compounds and biotech products . There are five chapters. Chapter 1: An introduction to chromatographic separations (1).A general description of chromatography (2).Migration rates of solutes (3).Zone broadening and colummn efficiency (4).Optimization of column performance (5).Summary of important relationships for chromatography (6).Applications of chromatography Chapter 2: Gas chromatography (1).Principles of gas liquid chromatography (2).Gas chromatographic columns and stationary phases (3).Applications of gas-liquid chromatography (GLC) Midterm test Chapter 3: High-performance liquid chromatography (1).Scope of HPLC (2).Column efficiency in liquid chromatography (3).Partition Chromatography Chapter 4:Capillary electrophoresis and capillary electrochromatography (1).An overview of electrophoresis (2).Capillary electrophoresis (3).Applications of capillary electrophoresis Chapter 5:PCR (1).Principles (2)Application of PCR Final Test |